Bbsi r0539s
WebWestlake Village, California 91361. Barrett Business Services Inc. 2555 Townsgate Road. Suite 120. Phone: 805-987-0331 Fax: 805-987-0365. AM: [email protected]. WebBbsI has a High Fidelity version BbsI-HF ® ( NEB #R3539 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more …
Bbsi r0539s
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WebCatalog NumberR0539L Concentration5 u/µL Quantity1500 units ApplicationsDNA Manipulation TypeRestriction Enzyme Temperature-80 C Sequence GAAGAC(2/6) Add to Compare List New England Biolabs 240 County Road Ipswich, Massachusetts1938 United States Phone: 800-632-5227 800-632-7799 978-921-1350 Company Profile Website: … WebR0539S / Lot: 10033032 Page 1 of 2 Product Name: BbsI Catalog Number: R0539S Concentration: 10,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Lot Number: 10033032 Expiration Date: 01/2024 Storage Temperature: -80°C
WebR0539S / Lot: 10055535 Page 1 of 2 Product Name: BbsI Catalog Number: R0539S Concentration: 10,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Packaging Lot Number: 10055535 Expiration Date: 09/2024 Storage Temperature: -80°C WebBbsI has a High Fidelity version BbsI-HF ® . High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward … An E. coli strain that carries the cloned and modified BbsI gene from Bacillus brevis. …
WebJun 18, 2024 · R0539S, Ipswich, MA, USA), 5 μL 10× reaction buffer (NEB, cat.# B7203S, Ipswich, MA, USA) and 43 μL ddH 2 O and was incubated at 37 °C for 2–3 h. Fifty microliters of vector was added to 150 μL... WebR0539S: 300 UNITS: 10,000 units/ml: £69.00: Time-Saver™ qualified for digestion in 5-15 minutes Used in Golden Gate Assembly. sgRNA Template Construction for CRISPR/Cas …
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the physician tale summaryWebBbsI has a High Fidelity version BbsI-HF ® ( NEB #R3539 ). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more … the physicist rightly dreadsWebCheck out videos that might be relevant for performing Restriction Enzymes BbsI using BbsI NEB#R0539 from New England BioLabs. Please note that these videos are … the physicists friedrich durrenmatt pdfWebR0539S / Lot: 10168571 Page 1 of 3 Product Name: BbsI Catalog Number: R0539S Concentration: 10,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in NEBuffer r2.1 in 1 hour at 37°C in a total reaction volume of 50 µl. Packaging Lot Number: 10168571 Expiration Date: 10/2024 the physicist\u0027s conception of natureWebR0539S / Lot: 10021730 Page 1 of 2 Product Name: BbsI Catalog Number: R0539S Concentration: 10,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Lot Number: 10021730 Expiration Date: 09/2024 Storage Temperature: -80°C the physicists daughterWebJul 12, 2024 · The oligos were phosphorylated by T4 Polynucleotide Kinase (M0201S, NEB) at 37°C for 30 min, then annealed by temperature ramping from 95°C to 25°C in a thermocycler. The PX330 backbone was digested with BbsI (R0539S, NEB) and ligated with the oligos using T4 ligase (15224017, Invitrogen). the physicist wife who overturned the worldWebBbsI (NEB, R0539S/L)3 µl. 10x NEB2.15 µl. dH2Oup to 50 µl. 2 Run the sample on 1% agarose gel, excise the linearized fragment and purify the DNA. 3 Quantify the purified DNA using NanoDrop and adjust concentration at 20 ng/µl. Ligation & transformation. 1 the physicists pdf