Fitc mouse igg1 κ isotype ctrl antibody
WebHost Mouse Isotype Mouse IgG1, κ Application FCM Storage Buffer PBS with 0.05% Proclin300, 1% Protein protectant ... Each lot of this antibody is quality control tested … WebAn isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry). We recommend FITC-conjugated mouse …
Fitc mouse igg1 κ isotype ctrl antibody
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WebThe MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues. WebFor flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 10 6 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for …
WebThe isotype control serves two purposes. Firstly, It would confirm the specificity of your primary antibody. Secondly, it will show you the fluorescence resulting from FC receptor mediated... WebPreparation And Storage. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with …
WebThe MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low … WebApplications Reported: FITC Mouse IgG1 K Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis. Applications Tested: This Mouse IgG1 K Isotype …
WebEach lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Web(555748) BD Pharmingen™ FITC Mouse IgG1, κ Isotype Control - BD Biosciences - CiteAb (555748) BD Pharmingen™ FITC Mouse IgG1, κ Isotype Control Sign in or register to save this reagent to your favourites Supplier BD Biosciences Host Mouse Type Primary Clonality Monoclonal (MOPC-21) Conjugate FITC Target Mouse Control IgG1 … raymond bartholomewWebThe antibodies react specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines. ... Mouse IgG1 FITC Regulatory Status Legend. RUO For Research Use Only. Not for use in diagnostic or therapeutic procedures. ... Isotype: Mouse BALB/c IgG1, κ Immunogen: Keyhole limpet hemocyanin (KLH ... simplicity children\u0027s patternsraymond basri morristownWebHuman peripheral blood lymphocytes were stained with anti-human CD3 APC and anti-human CD27 (clone S20020H) FITC (left) or mouse IgG1, κ FITC isotype control (right). See FITC spectral data Description CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. raymond basinWebIf you are using a mouse IgG1 monoclonal antibody that is conjugated to FITC, you should select a mouse IgG1 isotype control conjugated to FITC. As the fluorophore conjugation to the antibody (known as the F/P ratio) can vary between suppliers, it is best to purchase the isotype from the same supplier as the primary. raymond bassmastersWebIdeally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. simplicity childrens sewing machineWebMouse IgG1 kappa Isotype Control Antibodies Isotype control antibodies are similar to the primary antibody but are raised against an antigen that is not present in the experimental sample. They should match the host species, antibody class, and subclass, and conjugate of the primary antibody. simplicity children\\u0027s dress patterns