WebThe contents of the kit are sufficient for 50 coupling reactions using 200-500µg of protein per reaction. The kit has been optimized using purified IgG as the coupling protein. … WebThe cysteines at the N and C termini of proteins were coupled with 20–base pair (bp) maleimide single-stranded DNA oligos at 37°C for ... (2.1 μm, Spherotech) with anti-digoxigenin antibodies from Sigma-Aldrich using a carbodiimide reaction (PolyLink Protein Coupling Kit, Polysciences Inc.) The protein-coated beads were prepared by ...

Supporting Information - American Chemical Society

WebPolyLink Protein Coupling Kit #692. BioMag®Plus Mouse anti-Fluorescein IgG #704. Fluorescence Intensity Standards #711. ViaCheckTM Concentration Controls #721. BioMag® Maxi Carboxyl #722. BioMag® Maxi Amine #725. QuantumTM QC, 8 Peak Beads #725A. QuantumTM QC, 8 Peak Beads Supplement Histograms WebFluoresbrite microparticles and Polylink Protein Coupling kit for COOH microparticles were purchased from Polysciences, Inc. (Warrington, PA, USA). Antibodies: Abs specific for phospho-SHP-1, phospho-Syk and Akt were purchased from Cell Signaling Technology (Boston, MA, USA). the intelligent investor kindle

PDZ interactions regulate rapid turnover of the scaffolding protein ...

WebSpecialist distributor of life science reagents in the UK: PolyLink Protein Coupling Kit for COOH Microspheres, Diameter (PL01N-1KIT) by Bangs Laboratories WebMay 28, 2014 · Polysciences, Inc. offers the PolyLink Protein Coupling Kit for COOH. microspheres. The procedure that follows has been optimized for polymer. microspheres … Webprocedure of the PolyLink Protein Coupling Kit for COOH Microparticles (PolyScience, #24350) to covalently fix the beads and antibodies. ... Then, 0.17 mL of PolyLink coupling buffer included in the kit and 0.2 mL of goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody were added to each tube and the beads were suspended using voltex. the intelligent investor pdf google drive